Next Generation Sequencing (NGS)

 

BATJ offers a comprehensive range of next generation sequencing services (NGS) utilizing Thermo Fisher’s Ion PGM and Illumina’s Miseq and HiSeq 2500 platforms to deliver fast, reliable and high quality results. The services include:

 

A: Nucleic Acid Isolation Services 

BATJ offers high quality DNA/RNA isolation services from a wide range of sample types for various downstream applications such as PCR, qPCR, cloning, cDNA synthesis, DNA library construction, microarray, and NGS.

Sample types (gDNA, total RNA, mRNA, and miRNA, if applicable):

– FFPE tissue samples (blocks and/or slides)

– Microbiota samples

– Microbial cultures

– Human/animal cells and tissues

– Plant tissues

– Environmental samples

– Custom samples

Nucleic acid quality control: the quality of isolated DNA/RNA samples will be checked with either fluorometer or Bioanalyzer.

 

B: NGS Library Construction Services

BATJ has developed comprehensive high-quality NGS library construction protocols compatible to both Ion Torrent and Illumina platforms.

 Whole-Genome Sequencing (WGS)

– Plasmid and PCR Fragments Sequencing (PPS)

– Whole-Transcriptome Sequencing (RNA-seq)

– Targeted Sequencing (Gene Panels)

– Metagenomic Sequencing (16S/18S/ITS)

– Antibody Repertoire Sequencing (Ig-seq)

– Others

Library Quality Control: the quality of constructed NGS libraries will be examined with qPCR, fluorometer or Bioanalyzer.

 

C:   NGS with Thermo Fisher or Illumina’s machines for:

– Ampliseq ( 50-gene Cancer panel etc. );

– Metagenomic Sequencing (16s rRNA);

– Targeted Resequencing;

– SNP Discovery and Verification

– Genotyping By Sequencing; (GBS)

– Antibody Repertoire Sequencing (Ig-seq)

– Whole plasmids or PCR fragments service (PPS ) which was developed by BATJ that is a special low-cost and high-throughput. The advantages of PPS service over conventional ones are:

  1. No more primer walking for big size circular or linear DNA molecules in a single run, do not need to design and synthesis walking primers.

  2. Ideal for unknown plasmid structure or whole plasmid identification after modification.

  3. Suitable for a wide range of difficult templates (repetitive regions, strong secondary structures, GC rich.)

  4. Deep coverage of the whole DNA sequence (>100x).

  5. Up to 96 barcoded samples per chip run. Reference sequence preferred, but not required.

  6. Fast turnaround time (usually 2-3 business days).

***It is much better for de novo Whole Genome Sequencing (WGS), Whole Transcriptome Sequencing (RNA-Seq), mRNA-Seq, Metagenomic Sequencing (16s rRNA), Copy Number Analysis, DNA-Protein Interaction Analysis (ChIP-Seq), DNA Methylation analysis (DMA) with HiSeq-2500.

 

D: NGS Data Analysis Services

BATJ’s NGS data analysis pipelines combine and integrate most advanced bioinformatics tools for customized processing of your NGS data with analysis Pipelines:

– Sequence alignment (mutation detection)

– Gene panel data analysis (variant calling)

– Sequence assembly (de-novo or mapping)

– Metagenomic/Microbiome data analysis (16S/18S/ITS profiling)

– RNA-seq data analysis (differential gene expression analysis and pathway analysis)

– Antibody library analysis (CDR3 profiling)

– ChIP-seq data analysis

– DNA methylation data analysis

– For others services which is not listed here, please contact us.

 

E: NGS Data Report

     Demo of 50 Tumor Gene Analysis Report  at  Data Analysis Report 

                                                              ********************************

Detail of NGS service is on  Flyer of NGS Service . For more information, please Contact Us.

For your convenience, one “NGS Request Form” is available in Excel Format. It can be downloaded for easy editing at “ PGM_Request_Form-Excel version“. and “ MiSeq_Request_Form-Excel version“. Please download and fill out the form, then email or submit with your DNA samples.

More Services

  • DNA Sequencing (by Sanger)

    Long range (LR) DNA sequencing done with the newest version of the ABI 3730 capillary machine, readable bases up to 1,000 and longer. DNA sequencing price depends on the service....

    Learn More
  • Next Generation Sequencing (NGS)

      BATJ offers a comprehensive range of next generation sequencing services (NGS) utilizing Thermo Fisher’s Ion PGM and Illumina’s Miseq and HiSeq 2500 platforms to deliver ...

    Learn More
  • Fragment Analysis

    BATJ Inc.'s excellent protocol for fragment analysis is performed with 3730xl DNA Analyzer. Fragment Analysis Applications include: a. Microsatelite Analysis b. SNP Genotypi...

    Learn More
  • DNA Preparation

    DNA mini-prep with column (Qiagen) or gel clean method with Mini-Prep 96 instrument (MacConnell). The instrument uses a revolutionary new method of nucleic acid purification base...

    Learn More
  • Mutagenesis

    Site-directed mutagenesis for any combination of mutation within a 4 base-frame. The price includes quick change reaction, transformation, mini-prep and sequencing verification. ...

    Learn More
  • Primer Synthesis

    BATJ offers high quality longer oligos (up to 120 bases) for gene synthesis, cloning, mutagenesis quick change, primer walking sequencing, and PCR applications. Our synthesis mac...

    Learn More
  • Protein Expression (Bacterial)

    Cloning into Expression pET Vector: Clone (subclone) interest gene into a pET expression vector. The cloned gene will be verified by sequencing. The final editing sequence data ...

    Learn More
  • Fibercell System

    BATJ provide a best way to grow cells with Fibercell Systems. It can be used for large quantity production of Monoclonal antibodies, recombinant proteins, lymphocyte and virus. Fo...

    Learn More
  • Cloning and subcloning Service

    Clone an insert of interest into a vector provided by the customer. The cloned gene will be sequenced upon completion for verification. Sequencing data and mini-prep will be sent...

    Learn More